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Our aim is to construct physical clone maps covering those regions of chromosome 6 that are not currently extensively mapped, and use these to determine the DNA sequence of the whole chromosome. The strategy we are following involves establishing a high density framework map of the order of 15 markers per Megabase using radiation hybrid (RH) mapping. The markers are then used to identify large-insert genomic bacterial clones covering the chromosome, which are assembled into sequence-ready contigs by restriction enzyme fingerprinting and sequence tagged site (STS) content analysis. Contig gap closure is performed by walking experiments using STSs developed from the end sequences of the clone inserts. © 1997 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Original publication

DOI

10.3109/10425179709034066

Type

Journal article

Journal

Mitochondrial DNA

Publication Date

01/12/1997

Volume

8

Pages

151 - 154