Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We have quantified specific [3H]‐o uabain binding sites in normal human lymphocytes, and have measured the changes in the numbers of those sites which occur in response to various stimuli. We have confirmed previous findings that incubation for 72 h in the presence of fetal calf serum causes an increase in [3H]‐ouabain binding, and that this does not occur if the cells are incubated in fetal calf serum which has first been dialysed. During incubation of the lymphocytes for 3 days in the presence of dialysed fetal calf serum each of the following stimuli caused an increase in specific [3H] ‐ouabain binding: addition of ethacrynic acid (1 mumol l‐1), addition of lithium (1 mmol l‐1), and reduction of the external potassium concentration (to 0.75 mmol l‐1). By analogy with the similar results in HeLa cells reported by others, we suggest that the increase in [3H]‐ouabain binding may, in the case of ethacrynic acid and the reduction of the external potassium concentration, be initiated by an increase in the intracellular sodium concentration. The mechanisms whereby fetal calf serum and lithium cause an increase in [3H] ‐ouabain binding are not clear. 1986 The British Pharmacological Society

Original publication

DOI

10.1111/j.1365-2125.1986.tb02887.x

Type

Journal article

Journal

British Journal of Clinical Pharmacology

Publication Date

01/01/1986

Volume

22

Pages

275 - 279