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To help stem the growing tide of antimicrobial resistance, the United Nations General Assembly recently ratified new global targets for rational antibiotic use in humans. However, the tools needed to assess the cost-effectiveness of the interventions targeting more rational antibiotic use are lacking. The GAPi project is a Wellcome Trust funded research project focused on the development of modelling frameworks to predict the health and economic consequences of global antibiotic policy setting. GAPi brings together policy experts, health economists, and infectious disease modellers from key organisations and partners in low- and middle-income countries to generate evidence on the impacts and cost-effectiveness of interventions designed to help achieve global antibiotic use targets, including vaccination, rapid diagnostics and national medicines policies.